By Igor A. Kaltashov,Stephen J. Eyles
fresh advances in mass spectrometry (MS) have driven the frontiers of analytical chemistry into the biophysical laboratory. for that reason, the biophysical community's popularity of MS-based equipment, used to review protein higher-order constitution and dynamics, has sped up the growth of biophysical MS.
regardless of this turning out to be development, earlier no unmarried textual content has provided the complete array of MS-based experimental concepts and methods for biophysics. Mass Spectrometry in Biophysics expertly closes this hole within the literature.
masking the theoretical history and technical features of every approach, this much-needed reference deals an extraordinary assessment of the present kingdom of biophysical MS. Mass Spectrometry in Biophysics starts with a priceless dialogue of basic biophysical ideas and MS-related suggestions. next chapters address:
* smooth spectrometric hardware
* High-order constitution and dynamics as probed by way of numerous MS-based methods
* strategies used to check constitution and behaviour of non-native protein states that develop into populated below denaturing conditions
* Kinetic elements of protein folding and enzyme catalysis
* MS-based tools used to extract quantitative details on protein-ligand interactions
* Relation of MS-based innovations to different experimental instruments
* Biomolecular houses within the fuel phase
totally referenced and containing a worthy appendix at the physics of electrospray mass spectrometry, Mass Spectrometry in Biophysics additionally bargains a compelling examine the present demanding situations dealing with biomolecular MS and the aptitude functions that may most likely form its future.
Read or Download Mass Spectrometry in Biophysics: Conformation and Dynamics of Biomolecules (Wiley Series on Mass Spectrometry) PDF
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